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Description
Rat GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) ELISA KitThe Rat (GAPDH) Glyceraldehyde 3 phosphate Dehydrogenase ELISA Kit measures Glyceraldehyde 3 Phosphate Dehydrogenase in Rat samples. The plate has been pre coated with Rat GAPDH antibody. GAPDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GAPDH Antibody is added and binds to GAPDH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GAPDH antibody. After incubation unbound
The Rat (GAPDH) Glyceraldehyde-3-phosphate Dehydrogenase ELISA Kit measures Glyceraldehyde-3-Phosphate Dehydrogenase in Rat samples. The plate has been pre-coated with Rat GAPDH antibody. GAPDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GAPDH Antibody is added and binds to GAPDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GAPDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GAPDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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