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Description
Rat OPN (Osteopontin) ELISA KitThe Rat (OPN) Osteopontin ELISA Kit measures Osteopontin in Rat samples. The plate has been pre coated with Rat SPP1 antibody. SPP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SPP1 Antibody is added and binds to SPP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPP1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
The Rat (OPN) Osteopontin ELISA Kit measures Osteopontin in Rat samples. The plate has been pre-coated with Rat SPP1 antibody. SPP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SPP1 Antibody is added and binds to SPP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SPP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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