SKU: 92480104478

Human ATG12 (Ubiquitin-like protein ATG12) ELISA Kit

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Description

Human ATG12 (Ubiquitin-like protein ATG12) ELISA KitThe Human ATG12 (Ubiquitin like protein ATG12) ELISA Kit measures ATG12 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ATG12 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Human ATG12 (Ubiquitin-like protein ATG12) ELISA Kit measures ATG12 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ATG12 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ATG12 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ATG12 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 92480104478

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4.8 ★★★★★
Based on 1802 reviews
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Wisconsin Customer
Waukegan, US
★★★★★ 5
Work great, no morning groggy, made in USA
Flavor Name: Peppermint
These make me sleep like a baby! I am 52 and often could not turn my brain off to go to sleep or stay asleep at night. About 30-45 minutes after taking these my head hits the pillow and I’m out! I take 3 tablets / 15 mg. No groggy feeling in the morning. My previous experience with melatonin had been gummies - 10-12 mg and did not work as well. Taste was also inconsistent. These are a nice peppermint, like the circle-O candies we ate as a kid. A little chalky but not bad. They don’t dissolve as fast as I thought but directions say ok to chew so then they dissolve very quickly. I also like that company is fully USA for development and manufacture. Many of gummies are not. The tablet format is also very convenient to pack for travel. I have not tried full pill format by any manufacturer.
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Reviewed in the United States on March 18, 2021
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Verified Purchase
Tina
Alexandria, US
★★★★★ 5
Flavor is great, works well!
Flavor Name: Peppermint
Excellent when using 2, great flavor/taste. Thank you!
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Reviewed in the United States on November 29, 2025
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Lillie
San Leandro, US
★★★★★ 5
Like the dissolvable feature
Flavor Name: Peppermint
Cardiologist recommended one tablet nightly 30 minutes before bed to protect the lungs from covid. His other recommendations were standard protocol. Had never heard of this but know that melatonin is supposed to help with sleep. Had tried melatonin for sleep, at higher strength, but effect wore off after a week. Suprisingly, this seems to help with sleep as well. Large count at reasonable price.
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Reviewed in the United States on April 7, 2021
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Verified Purchase
Edward A. Rutherford
Lexington, US
★★★★★ 4
Sleep aid that helpd.
Flavor Name: Peppermint
I purchased these because I understand that the natural production of melatonin decreases drastically after the age of 40. I have been using another brand that much more expensive for a lower dosage. I read the other reviews and decided to give these a try. I have been taking them for about 30 days. I think, I have a good understanding of the pills. It appears that the other brand had a more consistent effect. They sublingual and you would feel drowsy after about 20 minutes. These are slow desolve and take effect after 30 minutes or so. My only issue was consistency. I would say that 90% of the time I would be drowsy. Once or twice a month they would have a minimal effect. They are a stronger dose, 5 mg versus 3 mg of the other product. They are less expensive and they work most of the time.
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Reviewed in the United States on May 16, 2024
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Nazmo
Boise, US
★★★★★ 5
No after taste
Flavor Name: Peppermint
Easy on digestive system
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Reviewed in the United States on July 16, 2025

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