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Description
Human TP53BP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Tumor Protein p53 Binding Protein 1 (TP53BP1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Tumor Protein p53 Binding Protein 1 (TP53BP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Tumor Protein p53 Binding Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tumor protein p53 binding protein 1 (TP53BP1), also known as P53 binding protein 1 or 53BP1, is a protein encoded by the TP53BP1 gene. This protein functions in DNA, selects double-strand break repair pathways, promotes non-homologous end joining (NHEJ), and restricts homologous recombination. It plays multiple roles in the DNA damage response, including promoting checkpoint signaling after DNA damage, acting as a scaffold to recruit DNA damage response proteins to damaged chromatin, and promoting the NHEJ pathway by restricting end resection after double-strand breaks. Diseases associated with it include microcephaly and chorioretinopathy, an autosomal recessive form of microcephaly and chorioretinopathy. Pathways involved include cell cycle checkpoints and the ATM signaling network in development and disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 1717 reviews
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Product Reviews
★★★★★ 5
Basic mouse that works - - for now.
Size: 1 Pack, Style: USB Receiver, Color: Blue
I ordered this mouse (in blue) to replace the recently deceased exact same model that I've used for the past 2 or 3 years. I like the simplicity of the M185 mouse and mostly the best bang for your buck! I caught this one on sale for less than a ten-spot, so I thought, "why not."
I'm hoping that this one will last a bit longer than my two previous M185 models, that averaged about 3 years each. This model seems plagued by a short life span and is predisposed to mysteriously and suddenly dying, without any obvious symptoms or warning of any kind - - you're working and clicking along, going about your day, when suddenly the cursor on the computer screen freezes and that's it. Nothing, zero, nada. Funny thing, I'm very careful not to abuse or drop the mouse, and keep a fresh battery installed and watch for leakage and all that stuff - - they just stop working, and I assume it is caused by some sort of inherent electronic fault in the circuitry of the previous M185's. Due to my loyalty to the brand and model (and since it was on sale) I decided to give it one more shot. They say that "third time's a charm".
I like the fact that the M185 is simple and uncluttered with extra buttons that I would never use for my purpose, which is simple web-browsing and the occasional text document production. The feel is solid, not heavy, but not cheap feeling, either. I'm used to the shape of this mouse, which to me is quite comfortable for long sessions, another reason why I kind of wanted to stay with this model. The buttons have an audible "click", which doesn't bother me at all, and the wheel moves smoothly and also has a clicking operation (which I rarely ever use). The overall surface of the mouse has a matte finish, but like my previous models I'm sure it will eventually wear shiny spots where my fingers reside and will attain a more "well-used" look in time. Connecting it was a breeze, too. I just plugged the dongle into a spare rear usb port of my desktop, and the mouse connected instantly, no problem. The dongle can be stored safely inside the battery compartment, so you can take the whole thing out of the house if needed for use elsewhere. The speed of 1000 dpi seems just right for my purposes. The one I ordered even came with its own AA Duracell battery, which I wasn't really expecting. This mouse also enters "sleep mode" when you don't use it for a while, and I can't say how many times I've got up and left it on all night. And that's not hard to do, as I'll explain next.
The only real negative to this mouse is the absence of any easily visible status indicator of it's on / off switch. The rear housing of this mouse is matte black, including the tiny switch where you turn it on and off with your fingernail, with only a very faint "on" lettering (also black), and very hard to see with my aging eyes. The laser is not lighted or visible when on, also adding to the confusion, especially in a darkened room. So, as a workaround, I took a tiny drop of green paint and placed it down inside the switch slider to visibly help indicate the "on" position, and likewise a dot of bright orange for the "off" position. Many of Logitech's more expensive models (and a few other manufacturers) include this color-coded switch indication. I guess for the $$, they had to cut corners somewhere - - a minor complaint.
In summary, this is a good basic mouse that works - - let's hope that this one will last longer than its two predecessors.
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Reviewed in the United States on February 17, 2023
★★★★★ 5
My favorite mouse ever
Style: Right-Handed, Pattern Name: Mouse, Color: Off White
I'm very particular when it comes to mice. It has to be:
- quiet click and scroll
- scroll wheel, not track ball
- bluetooth
- vertical
- real battery, NOT rechargeable
There are actually several mice that fit all those criteria, and I've tried nearly all. This Logitech is double the price of most of the others, but for me well worth it. Prior to this I used the Mojo which is about half the price, and that mouse is probably my 2nd favorite but it is a distant second. Compared to the Mojo and others, this mouse has more precise movement, smoother scrolling, and more reliable clicks. Because of the vertical orientation, my finger tends to click diagonally downward with gravity instead of fully horizontal in line with the intended click direction. That causes the Mojo to sometimes miss my clicks, forcing me to constrain my finger direction more carefully, often requiring several tries just to click something. But this Logitech has no such issue, it's very responsive every time even with my sloppy clicks.
So while I used to think the Mojo was the best I could find given my requirements, this Logitech is clearly better. When I spend all day at the computer, these minor things make a big difference to me, therefore well worth the extra money.
Also want to mention I'm 6' 4" tall and the size of this mouse is fine for me. It is a little smaller than some others, but it feels fine in my hand, it's not abnormally tiny or anything. I was concerned because the description says it's intended for smaller hands and suggested I should get their larger MX Vertical model instead. I would have considered it but the MX Vertical has a rechargeable battery which is a deal-breaker for me. What's my beef with rechargeable batteries? Earlier I had the Seenda vertical mouse but its rechargeable battery died after a year, unable to recharge, so I figured it was a fluke and bought another, and same thing happened after a few months. So after that I switched to replaceable batteries and never looked back. Replaceable batteries last forever (well, they only last a year too, but then you just replace the battery!). I've been very happy using replaceable batteries, way more reliable and durable, because all rechargeables die eventually, and it is easier to replace a battery than an entire mouse!
So in summary, if you're price sensitive you could get a usable vertical mouse for less. But if you're OK to spend a little more for a tool you might well use all day for years, then this one is totally worth it IMO.
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Reviewed in the United States on March 27, 2025
★★★★★ 5
Don't Delay - Get One
Style: Right-Handed, Pattern Name: Mouse, Color: Graphite
Make sure you get the right size - I got the Small/Med. Perfect fit. Have had tennis elbow for years and the computer use exacerbated the issue. Very comfortable, great fit. The best position for your hand is in the upright position. Would recommend one for everyone. Good, solid mouse.
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Reviewed in the United States on April 10, 2026
★★★★★ 5
Bad auto driver install. Great mouse. Few of the left hand mfgs.
Style: Left-Handed, Pattern Name: Mouse, Color: Graphite
Really crappy auto driver install. Locked up my USB ports for my usb keyboard, usb mouse, all usb ports in fact. Had to reboot multiple times with an old style 5pin keyboard since the software screwed up my usb ports. All of them. Lucky I have a motherboard with an old style keyboard port. A reboot of W11 in safe mode, ripped out auto-installed software, registry keys and rebooted to regain control of my USB ports. I do not know what went wrong. Alls I know is Logitech loaded something, it locked up my USB ports and I had to rip everything Logitech out to get control again. I downloaded the software, installed it and it worked fine. I've had no problems since.
I'm an old lefty and as such am ambidextrous in most areas, since there just was not left handed oriented items readily available. You had to have left handed stuff made so you learned to do things right handed. I've developed carpal tunnel in both elbows and had purchased a couple of vertical mice, one left and one right. I took the left into work and was looking for a replacement. My other two were around $30 ea. I liked the right handed vertical mouse (Anker). They do not make a left handed version. The left handed mouse is ok, not as good as the Anker. I decided to go above my spend tolerance and get the Logi after reading many reviews. It has not disappointed me. The auto-install sucked. Never letting that happen again.
It is a nice smooth mouse. Fine movements that are easily adjustable. The hand position is excellent. I can use this all day without switching to the right because of elbow pain. If you have the pain, you know what I am talking about when it flares. It takes some getting used to the subtlety of the clicks when coming from lower cost mice. Soft and gently like a newborn's ....
One of my big criteria for a vertical mouse is a landing area for the outer two fingers (pinky and next to pinky for finger orientation challenged). When you go to a vertical mouse, it takes some adjustment to moving the mouse since you can't really press down with your palm and inside two fingers (the ones next to the outside two fingers). It is helpful to be able to use the two outer fingers and thumb to aid in moving the mouse. You have to experience a vertical mouse to understand. The right handed only Anker goes from an inch to a half inch in the length of my pinky below the buttons on the side. This is a really nice landing area for the outside fingers to assist moving the mouse around. You can squeeze a little and the mouse glides. The Logi mouse is a wee shorter than the Anker and the area below the buttons in a mostly straight 3/4". Both mice curve quickly down to the inside below this area. I was used to a vertical mouse by the time I purchased the Logi and I have no issues with the pinky dragging the table and the finger next to pinky resting on the landing. I move the mouse with no problems. It is the subtlety of how one interacts with these style of mice. The big plus, takes a long time before mouse use causes pain flareups. And this isn't just a pain and done. Depending on how one ignores the pain, often determines how many days life sucks. The Logi mouse minimizes the flare ups. I'm thinking of taking the Anker in and getting a right handed Logi for home. I like the Anker, I'm frugal and highly recommend it for getting your feet wet. I love the Logi (auto-install sucks) and will probably ignore my frugal tendencies and get a right handed one since I don't switch hands as frequently with the Logi in the mix. Don't have to suffer auto-install either.
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Reviewed in the United States on February 29, 2024
★★★★★ 5
Pretty in Pink
Style: Right-Handed, Pattern Name: Mouse, Color: Rose, Style: Right-Handed, Pattern Name: Mouse, Color: Rose
Logitech is a great brand, so that is why I trusted in throwing down the money for this mouse. It feels great, easy to use and the battery life is wonderful! Good quality, abd was very easy to connect to my personal and work laptop!
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Reviewed in the United States on May 17, 2026
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