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Description
Human FⅧ ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Coagulation Factor VIII (FVIII) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Coagulation Factor VIII (FVIII) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Coagulation Factor VIII ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Factor VIII (FVIII) is an important blood clotting protein, also known as antihemophilic factor (AHF). Factor VIII is encoded by the F8 gene. Defects in this gene cause hemophilia A, a recessive X-linked blood clotting disorder. Factor VIII is produced in the sinusoidal cells of the liver and the endothelial cells outside the liver, distributed throughout the body. This protein circulates in the bloodstream in an inactive form, bound to another molecule called von Willebrand factor, until an injury occurs that damages blood vessels. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.6 ★★★★★
Based on 2230 reviews
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Product Reviews
★★★★★ 5
The Ultimate Puzzle for Soccer Fans – Beautiful, Challenging & Totally Frame-Worthy ⚽🧩
Color: Wa 26 Ball-200, Size: 200Pcs
As someone who absolutely loves puzzles and is a huge World Cup fan, this one felt like it was made just for me. It combines two of my favorite things—a fun, satisfying puzzle experience and meaningful, display-worthy art—and honestly, it exceeded my expectations.
The design is stunning. The “We Are 26” FIFA World Cup theme gives it that special, collectible feel, especially knowing the 2026 tournament will be hosted across the U.S., Canada, and Mexico. It feels like a piece of history in the making, and that makes completing it even more exciting.
The 200-piece count is perfect for a relaxing but engaging session. It’s not overwhelming, but still gives you that satisfying challenge. I loved how immersive it felt—great for unwinding without staring at a screen, and it really keeps your mind focused and engaged.
What truly sets this puzzle apart is the quality of the wooden pieces. They’re thick (5mm!) and durable, with a really nice weight to them. They fit together beautifully and feel sturdy enough that once completed, the puzzle holds its shape—which is ideal if you plan to display it.
And yes… this is absolutely frame-worthy. The finished 11.8 x 11.8 inch size is perfect for wall décor, and the design looks so clean and polished once complete. I love turning my puzzles into art, and this one looks amazing in a home office or living space—especially for anyone who loves soccer.
Why I love it:
* Official FIFA World Cup 2026 design with real collectible value
* Perfect balance of challenge and relaxation
* Thick, high-quality wooden pieces that feel durable and premium
* Beautiful finished look—ideal for framing or gifting
* Great for puzzle lovers and soccer fans alike
Perfect for gifting: I’ve already decided this will be one of my go-to gifts for friends and family who love soccer. It’s thoughtful, unique, and something they can enjoy and keep.
Final thoughts: If you love puzzles that you can complete, admire, and proudly display, this one is a standout. And if you’re also a World Cup fan? It’s truly the best of both worlds. ⚽✨
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Reviewed in the United States on April 25, 2026
★★★★★ 5
Premium Quality, Beautiful Puzzle
Color: Usa Ball-200, Size: 200Pcs, Color: Usa Ball-200, Size: 200Pcs
I will preface this by saying that I am not usually a big fan of puzzles. Puzzles I’ve done in the past are too flimsy and there’s been too much guesswork about whether pieces actually go together. But As a huge fan of the World Cup, this puzzle was a must-have. It far exceeded my expectations! The craftsmanship is outstanding. Each of the pieces is made from thick, high-quality wood (the 5mm thickness really makes a difference), giving it a premium feel that you don’t get with standard cardboard puzzles. There’s a satisfying feel when 2 pieces go together. It actually felt like an accomplishment to finish the whole thing. The fact that it comes with a backing that allows you to permanently fix it in place and display it is another added touch that I loved. It’s just under 12 inches in diameter once complete, which looks great on the wall if you decide to hang yours once finished. I think it’s a great value when compared to standard cardboard puzzles. You can tell that there is actually a lot of thought placed into creating this puzzle.
What really sets this puzzle apart are the unique piece shapes. There are about 10 pieces that are made in themed shapes, like the Statue of Liberty, soccer players, a cleat, etc. Somehow, despite these pieces being there, the puzzle was still a challenge which took me about 2 1/2 hours to finish.
This would make an amazing gift for any soccer fan or puzzle enthusiast, especially with the excitement building around the 2026 tournament.
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Reviewed in the United States on March 24, 2026
★★★★★ 4
Officially Licensed, Officially Priced!
Color: Trophy 3, Size: 200Pcs, Color: Trophy 3, Size: 200Pcs
Let me first state that this is a nice package. It's officially licensed, includes everything you need to convert it to make it a permanent decoration (or at least an every four years decoration), and is of very high quality.
The pieces are really cool, I love the variety and the recognizable items. I really only have two complaints:
1. The price. It says FIFA, so you pay for it. This is a high price for a wooden puzzle of this size, as they are usually at least $10 less, plus it's always a bummer giving FIFA any more money.
2. There is a VERY strong chemical aroma, which will hopefully dissipate. The puzzle is shipped in plastic, so hopefully a little more time out of the plastic will improve that aspect.
Overall it's a fun puzzle. I found the black around the trophy to be a bit challenging, but the crazy shapes of the pieces make it easier than it would be with standard puzzle pieces. Plus the unique recognizable pieces help!
I'll give it a solid 4/5 despite my disdain for FIFA and the premium price - that is after-all justifiable to some degree given that we're a couple of months away from the World Cup. The one star deduction is purely down to the chemical aroma.
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Very well made but strong wood smell
Color: Trophy 3, Size: 200Pcs, Color: Trophy 3, Size: 200Pcs
This is a really well made puzzle. These pieces are nice and thick, the laser cutting is extremely precise, and the picture is stuck on very well to each one without peeling. There is an extremely strong burned wood smell when you open the box, but it's not a bad smell to me, more like a fireplace wood burning smell. Anyone that is very affected by strong smells would probably not like this puzzle though. I also don't know how long it will take for the smell to go away, if ever, but I've had this puzzle for 2 weeks now and the box still smells strongly like wood that has been set on fire.
We haven't put the puzzle together yet, life is just been rather busy, but the couple pieces I found that did fit together went very smoothly. I like how unique the shapes are, and that there are the World Cup icons as well. This should be a lot of fun!
It did come with a clear sticker to keep it together for hanging, but because of how that sheet was folded in the box, it'll have to be attached on the back or else there will be visible seams.
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Reviewed in the United States on April 12, 2026
★★★★★ 5
Official FIFA Product
Color: Trophy 3, Size: 200Pcs, Color: Trophy 3, Size: 200Pcs
This is not my 1st Woodlux puzzle. They are well made, unique puzzles with laser cut pieces that are very precise. The FIFA 2026 World Cup Trophy puzzle is no different. This puzzle has large swaths of solid black which would seem difficult, but the uniqueness of the pieces gives you more than one way to figure out where the pieces go. Colors on the puzzle pieces are vibrant. There is a slight fire smell to the pieces, likely from the laser cutting. I found the smell to be noticeable, but not overpowering. Could be a problem for sensitive noses. 15 of the 200 pieces are soccer/FIFA shapes. This puzzle comes with a cotton drawstring bag to hold the pieces, as well as a cheat sheet, and sticky clear sheet to lay over the completed puzzle to display it as art. The assembled size of the puzzle is 9.2" x 13". This is an officially licensed FIFA product.
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Reviewed in the United States on April 18, 2026
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