Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein) ELISA Kit

Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein) ELISA KitThe Human BCL2L1 BCL X (Bcl 2 Like Protein 1 Bcl2 Associated X Protein) ELISA Kit measures BCL2L1 in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BCL2L1 BCL X antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human BCL2L1/BCL-X (Bcl-2 Like Protein 1/Bcl2 Associated X Protein) ELISA Kit measures BCL2L1 in Human Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BCL2L1/BCL-X antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with BCL2L1/BCL-X bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of BCL2L1/BCL-X in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.