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Description
Rat IL22Ra2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 22 Receptor Alpha 2 (IL22Ra2) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Interleukin 22 Receptor Alpha 2 (IL22Ra2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 22 Receptor Alpha 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | IL22RA2 is a secreted, soluble, monomeric protein that exhibits inhibitory properties against the effects of IL-22 in vitro. It belongs to the type II cytokine receptor family. It is a protein-encoding gene that encodes a member of the type II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits the activity of interleukin-22 by blocking its interaction with its cell surface receptor. The encoded protein may be important in regulating inflammatory responses and has been implicated in the regulation of colon tumorigenesis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 1980 reviews
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Product Reviews
★★★★★ 5
Style and comfort doesn't always have to be expensive:
Size: 13, Color: Wingtip Black
I bought this ones mainly because I have used them before and they are great for dancing. Specially salsa dancing. They look really stylish with formal cloth. A great blend of classic "wing tip" with modern details and smooth lines. They fit nice with thin and medium socks and have medium arc support. They will feel better with each use and are very good for salsa ballroom dancing turns on the forefoot and ruber on the heel for safe stops and stability. They fit true to size and foot stays locked in place with the lacing it provides. Great presentation with the box and awsome price for great quality formal shoes.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Bruno Moda Italy Men's Prince Classic Oxford Shoes - A Timeless Blend of Elegance and Comfort
Size: 12 Wide, Color: Brown
My experience with Bruno Moda Italy Men's Prince Classic Modern Formal Oxford Wingtip Lace-Up Dress Shoes has been truly exceptional, and I am delighted to share my positive review of these shoes that seamlessly marry classic style with modern comfort.
Timeless Design:
The standout feature of the Bruno Moda Italy Men's Prince Classic Oxford Shoes is their timeless design. The wingtip lace-up style, coupled with meticulous detailing and a classic silhouette, exudes a sense of sophistication and elegance. These shoes are a perfect embodiment of timeless formal fashion.
Craftsmanship Excellence:
The craftsmanship of these Oxfords is nothing short of excellent. The attention to detail, from the precisely stitched wingtip patterns to the well-finished lace-up closure, showcases the dedication to creating a high-quality and aesthetically pleasing pair of formal shoes. The use of premium materials adds to the overall luxurious feel.
Comfortable Fit:
Beyond their stylish exterior, the Bruno Moda Italy Oxfords offer a surprisingly comfortable fit. The interior is lined with a soft and breathable material, and the cushioned insole provides ample support. This thoughtful approach to comfort ensures that these shoes are not only eye-catching but also wearable for extended periods without sacrificing comfort.
Versatile Style:
These Oxfords effortlessly transition between classic and modern styles. Whether paired with a tailored suit for a formal event or worn with smart-casual attire for a sophisticated everyday look, the Bruno Moda Italy Men's Prince Classic Oxfords adapt seamlessly to various wardrobe choices. Their versatile style makes them a wardrobe staple for any well-dressed man.
Durable Construction:
Durability is a key feature of these formal shoes. The high-quality construction, including a sturdy sole and well-sewn seams, ensures that they withstand regular use without showing signs of wear and tear. The durability of these Oxfords adds to their overall value and longevity.
Secure Lace-Up Closure:
The secure lace-up closure of the Bruno Moda Italy Oxfords not only contributes to their classic look but also provides a secure and adjustable fit. The laces allow for customization based on individual foot shapes and preferences, ensuring a snug fit that enhances both comfort and stability.
Affordable Luxury:
Considering their impeccable design, craftsmanship, comfort, versatility, and durability, these Oxfords offer an affordable avenue for indulging in luxury footwear. The reasonable price point makes them an accessible choice for individuals seeking a pair of formal shoes that exude elegance without a hefty price tag.
Overall Satisfaction:
In conclusion, the Bruno Moda Italy Men's Prince Classic Modern Formal Oxford Wingtip Lace-Up Dress Shoes have exceeded my expectations with their timeless design, craftsmanship excellence, comfortable fit, versatile style, durable construction, secure lace-up closure, and affordable luxury. These Oxfords have become my go-to choice for formal occasions, impressing not only with their appearance but also with their comfort and durability. Highly recommended for men who appreciate the perfect blend of classic style and modern comfort in their formal footwear. Elevate your formal wardrobe with the Bruno Moda Italy Men's Prince Classic Oxfords!
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Reviewed in the United States on March 7, 2024
★★★★★ 4
Nice shoes for about a year and a half
Size: 13 Wide, Color: Brown, Size: 13 Wide, Color: Brown
I bought these shoes about a year and a half ago and they are comfortable and look much more expensive than they actually are. Today, I noticed them starting to tear a little bit, but if I can replace them every 18 months for this price, it is probably still a better value than high end shoes. The grip is good for dress shoes and has held up well, and they are a good fit that does not cause blisters from excessive rubbing. I might not choose these if I need to wear them every day because of their lifespan, but for a once or twice a week pair of dress shoes, they are a good value.
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Reviewed in the United States on May 25, 2025
★★★★★ 5
Runs big but great shoe!
Size: 12 Wide, Color: Black
Great looking shoe at a very good price! Runs big, I had to get the next size down for my son.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Fantastic Shoe for the Price
Size: 9.5 Wide, Color: Wingtip Black
Okay, I’m used to spending big bucks on men’s dress shoes. I’m talking $300 plus for American made shoes. They are recraftable (resole-able) for another $100 each time.
But since Covid, I don’t go to the office as often. One of my black dress shoes needs to be re-soled, and it will be. Just not now.
So I ran into these shoes on Amazon. It’s hard to argue with the price. Literally 10% of what I have been paying. So, are these shoes 90% lower in quality?
The short, simple answer is, surprisingly, no. Just the opposite.
Upon receipt, I inspected them carefully and they looked pretty darned decent. I was looking for any excuse to plop them back in the box and return them. I couldn’t find anything.
I appreciate that the leather upper is smooth and mildly shiny. This is a wing tip style shoe, so I’m impressed that the extensive stitching was evenly spaced. The leather detailing that I expect on a wing tip is also all there.
Inside the shoe, it is lined from the toes all the way to the heel box… nice! And that leather feels surprisingly supple. That is something I would expect on a much more expensive shoe. But definitely not at this price point.
Hmm.
I even liked the wax laces they chose. They are a little thicker and have more than adequate length. The shoe’s eyelets have interior facing metal grommets to protect against stretching and tear out of the leather and helps protect the laces against friction wear, as they are tightened and loosened over time. Now having that detail, at this price point, has raised both of my eyebrows.
Double hmm. Now I am really impressed.
The only thing that I didn’t like was that the stitching—that joins the uppers to the sole—is white. I needed to polish these shoes anyway, so I figured I’d see what would happen if I put black polish on the stitching.
I’m happy to say that the uppers shine up very nicely, to a high gloss. This was done with just a little bit of buffing, using a traditional horse hair bristled brush. And followed by a buffing cloth. Although I didn’t do it, the leather is smooth enough that I probably could have spit shined them.
And those pesky white stitches on the top side of the soles? Well I’m happy to report that they are now definitely black.
Turning the shoe over, I can visually see a lump where the shank is supposed to be. That’s a good thing. Not all shoes have a shank. It helps to support the arch. That’s another nice detail. I am expecting the shank to be plastic at this price point. So I am utterly shocked to report to you that a magnet sticks to the bottom of the shoe. That shank is steel! Now I am amazed! We’re talking mind blown, Purple smoke everywhere.
Speaking of details, I was very surprised to see the nicely done wing tip detailing, from on top of the shoe, carried over to the bottom of the sole. Wow. I have never seen that before. That’s surprising.
Now it is important to point out that the sole is some man made material. Definitely not leather. But that’s okay. Especially in the wet, leather wears out quickly, if you don’t wear rubber over shoes. One less thing to fuss about.
The heel itself has a band/layer of rust brown material sandwiched between black layers. I wonder if that is a cork or bamboo material? Having walked in these shoes, on concrete and tile floors, last week, there is a noticeable level of cush, or give. They will definitely not to be confused with sneakers. But for dress shoes, this is a pleasant and surprising experience.
The first day, I wore them at home, over the weekend and I’m glad I did that. They needed time to adjust to my feet. Any dress shoe that I have ever worn needs that.
But after that, these shoes have been pleasant enough to walk around in at work.
I’d have to say that these shoes give me at least 90% of what I would expect from my $300+ shoes for around 10% of the cost.
In my estimation, at their current price point, they constitute an excellent deal.
And especially if you are just breaking into the business world and need a good pair of interview shoes, these are definitely respectable… just be sure to put black polish on the white stitches.
Oh, BTW, I have wider feet and I ordered the shoe wide. After the initial stretching, they fit nicely.
So, in the final analysis, what do I think? I have to say, yes, these shoes definitely get my recommendation. Especially if price is a huge factor, then these shoes constitute an excellent deal.
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Reviewed in the United States on March 20, 2023
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